Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
In 1x PBS, pH 7.4
Handling Advice
Aliquot to avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Fulka, Langerova: "The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition." in: Development (Cambridge, England), Vol. 141, Issue 8, pp. 1694-704, (2014) (PubMed).
Østrup, Strejcek, Petrovicova, Lucas-Hahn, Morovic, Lemme, Petersen, Laurincikova, Niemann, Laurincik, Hyttel: "Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle." in: Cellular reprogramming, Vol. 13, Issue 2, pp. 145-55, (2011) (PubMed).
Østrup, Petrovicova, Strejcek, Morovic, Lucas-Hahn, Lemme, Petersen, Niemann, Laurincik, Maddox-Hyttel: "Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryos." in: Cloning and stem cells, Vol. 11, Issue 3, pp. 367-75, (2009) (PubMed).
Song, Lee, Kim, Kim, Park, Kim, Chang, Han, Lee, Lee, Koo: "Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells." in: BMC developmental biology, Vol. 9, pp. 44, (2009) (PubMed).
Target
UBTF
(Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))