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MAD2L1 antibody (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast))

Details for Product anti-MAD2L1 Antibody No. ABIN567786, Supplier: Log in to see
Antigen
  • AA673185
  • cb408
  • HSMAD2
  • MAD2
  • mad2
  • MAD2L1
  • XMad2
  • zgc:112133
Reactivity
Human, Mouse (Murine), Rat (Rattus)
118
29
27
4
4
4
2
2
1
1
Host
Mouse
72
32
14
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MAD2L1 antibody is un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
102
46
23
21
20
19
12
6
2
1
1
1
1
1
Supplier
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Immunogen Recombinant Human MAD2.
Clone 2G9
Isotype IgG1
Specificity This antibody reacts with MAD2 (25 kDa) on Western blotting and Immunoprecipitation.
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Alternative Name MAD2L1 (MAD2L1 Antibody Abstract)
Background Some of the spindle checkpoint components are evolutionarily conserved from yeast to human. They include mitotic arrest deficiency 1 (MAD1), MAD2, MAD3/BUBR1, budding uninhibited by benomyl 1 (BUB1), BUB3, and monopolar spindle 1 (MPS1). MAD1 interacts with several checkpoint proteins, forming a complex with MAD2 and recruiting MAD2 to unattached kinetochores. MAD1 and Cdc20 can bind MAD2, but these two bindings are exclusive. They share a 10-residue MAD2-binding motif. It has been suggested that MAD1 acts as a positive regulator and a competitive inhibitor of the MAD2-Cdc20 complex.Synonyms: MAD2, Mitotic spindle assembly checkpoint protein MAD2A
Gene ID 4085
NCBI Accession NP_002349
UniProt Q13257
Research Area Cell Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-MAD2L1 antibody (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast)) (ABIN567786) anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibody
Western Blotting (WB) image for anti-MAD2L1 antibody (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast)) (ABIN567786) anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibody (Image 2)
Background publications Wei, Chou, Ou, Yeh, Tyan, Sun, Shen, Shieh: "TTK/hMps1 participates in the regulation of DNA damage checkpoint response by phosphorylating CHK2 on threonine 68." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 7748-57, 2005 (PubMed).

Sudakin, Chan, Yen: "Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2." in: The Journal of cell biology, Vol. 154, Issue 5, pp. 925-36, 2001 (PubMed).