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Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody

Details for Product No. ABIN567796, Supplier: Log in to see
Antigen
  • Caspase-12
Epitope
N-Term, AA 95-318
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14
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11
11
9
7
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2
2
2
2
2
1
1
1
1
1
1
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1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
99
86
61
Host
Rat
126
11
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
125
59
50
36
15
12
11
7
5
4
2
1
Supplier
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Immunogen Recombinant Caspase-12 N-terminal fragment (aa 95-318).
Clone 14F4
Isotype IgG2a
Specificity This antibody reacts with Caspase-12 (~53 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.
Purification Protein-G Agarose Chromatography of hybridoma supernatant.
Alternative Name Caspase-12 (CASP12 Antibody Abstract)
Background Caspase-12 is localized to the endoplasmic reticulum (ER), but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis and accumulation of excess proteins in ER, but not by membrane- or mitochondrial-targeted apoptotic signals. One well-known cause of ER stress in neurons is the accretion of amyloid-beta, the insoluble protein that forms plaques in the brains of patients with Alzheimer's disease. Caspase-12 mediates cytotoxicity induced by amyloid-beta and ER stress-induced apoptosis. Caspase-12 is ubiquitously expressed in tissues. Similar to other members of the Caspase family, Caspase-12 requires cleavage of the prodomain to activate its proapoptotic form.Synonyms: CASP-12, CASP12, Inactive caspase-12
Gene ID 100506742
NCBI Accession NP_001177945
UniProt Q6UXS9
Research Area Apoptosis/Necrosis
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody (ABIN567796) anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody
Western Blotting (WB) image for anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody (ABIN567796) anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody (ABIN567796) anti-Caspase 12 (CASP12) (AA 95-318), (N-Term) antibody (Image 3)
Background publications Morishima, Nakanishi, Takenouchi et al.: "An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34287-94, 2002 (PubMed).

Nakagawa, Yuan: "Cross-talk between two cysteine protease families. Activation of caspase-12 by calpain in apoptosis." in: The Journal of cell biology, Vol. 150, Issue 4, pp. 887-94, 2000 (PubMed).

Nakagawa, Zhu, Morishima et al.: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).