Glucuronidase beta antibody (Biotin)

Catalog No. ABIN568042

Product Details anti-Glucuronidase beta Antibody (Biotin)

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucuronidase beta antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG~ 6.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Alternative Name: beta-Glucuronidase (GUSB Products)

Synonyms: GUSB antibody, beta-GUS antibody, gus antibody, BG antibody, MPS7 antibody, AI747421 antibody, Gur antibody, Gus antibody, Gus-r antibody, Gus-s antibody, Gus-t antibody, Gus-u antibody, Gut antibody, asd antibody, g antibody, Ac2-223 antibody, si:ch211-160e1.7 antibody, si:ct573103.7 antibody, glucuronidase beta antibody, beta-D-glucuronidase antibody, beta-glucuronidase antibody, beta glucuronidase antibody, glucuronidase, beta antibody, GUSB antibody, bglR antibody, SSO_RS14735 antibody, Glu antibody, Gusb antibody, gusb antibody

Background: Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB

Gene ID: 946149

NCBI Accession: AP_002238

UniProt: P05804

Pathways: Glycosaminoglycan Metabolic Process