Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
Specificity
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phosphomannose isomerase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Characteristics
Molar Ratio: Biotin/IgG ~5.5
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phosphomannose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.