Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Molar Ratio: Biotin/IgG ~5.2
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Uridine-5’-Diphosphoglucose Dehydrogenase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
UGDH
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1B7
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.Synonyms: UDP-Glc dehydrogenase, UDP-GlcDH, UDP-glucose 6-dehydrogenase, UDPGDH