CLCA1 antibody (AA 872-884)

Catalog No. ABIN574037

Product Details anti-CLCA1 Antibody

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Binding Specificity: AA 872-884

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLCA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CLCA1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Marmoset (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide PETPSPDETSAPC from an internal region of human CLCA1 (NP_001276.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Marmoset (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL. Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx. 100kD band observed in Human Kidney and Lung lysates (calculated MW of 100kD according to NP_001276.2). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CLCA1

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Alternative Name: GOB5 / CLCA1 (CLCA1 Products)

Synonyms: CACC antibody, CACC1 antibody, CLCRG1 antibody, CaCC-1 antibody, GOB5 antibody, hCLCA1 antibody, hCaCC-1 antibody, Clca3 antibody, fi39d11 antibody, wu:fi39d11 antibody, AECC antibody, Clca1 antibody, Clca2 antibody, gob-5 antibody, gob5 antibody, chloride channel accessory 1 antibody, chloride channel antibody, chloride channel protein antibody, CLCA1 antibody, Clca1 antibody, clca1 antibody, clcA1 antibody

Background: Name/Gene ID: CLCA1
Subfamily: Chloride channel - calcium-activated CLC
Family: Ion Channel

Synonyms: CLCA1, CaCC-1, CLCRG1, HCLCA1, HCaCC-1, GOB5, CaCC, CACC1, Chloride channel accessory 1, Chloride channel regulator 1

Gene ID: 1179

NCBI Accession: NP_001276

UniProt: A8K7I4