AKT1 antibody (AA 2-480)

Catalog No. ABIN602721

Product Details anti-AKT1 Antibody

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Binding Specificity: AA 2-480

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.

Purification: Protein G purified

Sterility: Sterile filtered

Immunogen: Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (50 μg/mL), ICC (8 - 25 μg/mL), WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Suitable for use in Flow cytometry: 50 μg/mL. This antibody was validated for flow cytometry using MCF-7 cells. For. intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 20 μLL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25 μg/mL to detect Akt1 in fixed human peripheral blood mononuclear cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from 0.2 um sterile-filtered solution, PBS, 5 % trehalose

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1 / AKT (AKT1 Products)

Background: Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect