GNAT3 antibody (AA 304-318)

Catalog No. ABIN605025

Product Details anti-GNAT3 Antibody

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Binding Specificity: AA 304-318

Reactivity: Human, Mouse, Rat, Dog, Monkey, Pig, Rabbit, Cow, Hamster, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This GNAT3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GNAT3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Bat, Lizard (93%) Platypus (87%) Turkey, Chicken (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KNQFLDLNLKKEDKE from an internal region of human GNAT3 (NP_001095856.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Bat, Lizard (93%), Platypus (87%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 27kD band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kD according to NP_001095856.1. The 27kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GNAT3

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Alternative Name: GNAT3 / Gustducin (GNAT3 Products)

Synonyms: GDCA antibody, Ggust antibody, Gtn antibody, gnat-3 antibody, G protein subunit alpha transducin 3 antibody, guanine nucleotide binding protein, alpha transducing 3 antibody, GNAT3 antibody, Gnat3 antibody

Background: Name/Gene ID: GNAT3

Synonyms: GNAT3, Gustducin, Gustducin alpha-3 chain, GDCA

Gene ID: 346562

NCBI Accession: NP_001095856

UniProt: A8MTJ3

Pathways: Peptide Hormone Metabolism, G-protein mediated Events, Phototransduction