MAPK10 antibody (AA 25-74)

Catalog No. ABIN6746529

The Rabbit Polyclonal anti-MAPK10 antibody has been validated for WB. It is suitable to detect MAPK10 in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig and Xenopus laevis. There are 2+ publications available.

Quick Overview for MAPK10 antibody (AA 25-74) (ABIN6746529)

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPK10 Antibody

Binding Specificity: AA 25-74

Specificity: Rat MAPK10 / JNK3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa25-74 of rat Mapk10 (B0VXR6, NP_036938). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%), Orangutan, Sea squirt, Drosophila, Nematode (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-MAPK10 antibody (ABIN6746529)

Deaglio, Morra, Mallone, Ausiello, Prager, Garbarino, Dianzani, Stockinger, Malavasi: "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 395-402, (1998) (PubMed).

Konopleva, Estrov, Zhao, Andreeff, Mehta: "Ligation of cell surface CD38 protein with agonistic monoclonal antibody induces a cell growth signal in myeloid leukemia cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4702-8, (1998) (PubMed).

Target Details for MAPK10

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Alternative Name: MAPK10 / JNK3

Background: Name/Gene ID: MAPK10
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK10, C-Jun N-terminal kinase 3, JNK3, JNK3A, MAPK 10, MAP kinase 10, MAP kinase p49 3F12, p493F12, p54B SAPK, SAPK beta, p54bSAPK, SAPK1b, Serk 2, JNK3 alpha protein kinase, PRKM10

Gene ID: 5602

NCBI Accession: NP_036938

UniProt: P53779

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades