PDE8A antibody (AA 551-600)

Catalog No. ABIN6746531

This Rabbit Polyclonal antibody specifically detects PDE8A in WB. It exhibits reactivity toward Human, Rat, Monkey, Rabbit, Dog, Horse, Cow and Guinea Pig and has been mentioned in 4+ publications.

Quick Overview for PDE8A antibody (AA 551-600) (ABIN6746531)

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Reactivity: Human, Rat, Monkey, Rabbit, Dog, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE8A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDE8A Antibody

Binding Specificity: AA 551-600

Specificity: Human PDE8A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Mouse, Rat, Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa551-600 of human PDE8A (O60658, NP_002596). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%), Mouse, Elephant, Pig, Opossum, Turkey, Zebra finch, Chicken, Xenopus, Zebrafish (92%), Marmoset, Bat (85%), Platypus, Drosophila (84%), Bloom alga (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PDE8A antibody (ABIN6746531)

Shahan, Sorenson, Simpson, Kefalides, Lewis: "Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10175-81, (2000) (PubMed).

Yang, Malek, Desiderio: "An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo." in: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20832-40, (1995) (PubMed).

Aoki, Isselbacher, Pillai: "Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10606-9, (1994) (PubMed).

Vetrie, Vorechovský, Sideras, Holland, Davies, Flinter, Hammarström, Kinnon, Levinsky, Bobrow: "The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases." in: Nature, Vol. 361, Issue 6409, pp. 226-33, (1993) (PubMed).

Target Details for PDE8A

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Alternative Name: PDE8A

Background: Name/Gene ID: PDE8A
Subfamily: PDE - cAMP-specific, high affinity
Family: Phosphodiesterase

Synonyms: PDE8A, Phosphodiesterase 8A, HsT19550

Gene ID: 5151

NCBI Accession: NP_002596

UniProt: O60658

Pathways: cAMP Metabolic Process