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Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594)

Antigen

IgG
Binding Site
Alternatives

Heavy & Light Chain
Host
Alternatives

Donkey
Reactivity
Conjugate
Alternatives DyLight 594
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
2 references available
Catalog no. ABIN611731
Quantity 1 mg  (1 mg/ml (E 1% at 280 nm = 13.0))  (Variants)
Price 171.29 $   Plus shipping costs $45.00
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Immunogen Purified Goat IgG, whole molecule
Format Lyophilized
Description Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight 594 Conjugate, min x w/human, mouse, rabbit or rat IgG. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm). Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Comments

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details
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Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Concentration 1 mg/ml (E 1% at 280 nm = 13.0)
Purity Affinity purified antibody is > 95% based on SDS-PAGE
Purification Affinity purified using solid phase mouse IgG (H&L)
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide
Storage Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.
Research Area Secondary Antibodies
Restrictions For Research Use only

Publications
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Product Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).

Martinez-Exposito, Kaplan, Copeland et al.: "Retention of the BUB3 checkpoint protein on lagging chromosomes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8493-8, 1999 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (3758), Rabbit (2825), Donkey (1019), Chicken (371), Mouse (297), Sheep (199), Guinea Pig (29), Rat (20), Pig (14), Duck (6), Horse (5), Cat (Feline) (2), Hamster (1), Human (1)
Reactivities Mouse (Murine) (1409), Human (1282), Rabbit (1144), Rat (Rattus) (952), Goat (703), Guinea Pig (388), Sheep (Ovine) (340), Cow (Bovine) (273), Pig (Porcine) (185), Cat (Feline) (184), Dog (Canine) (179), Chicken (177), Horse (Equine) (170), Hamster (128), Donkey (75), Monkey (70), Golden Syrian Hamster (69), Syrian Hamster (62), Llama (51), Armenian Hamster (27), Mink (18), Ferret (13), Turkey (13), Bird (Avian) (6), Duck (5), Rhesus Monkey (5), Deer (4), Pigeon (3), mouse (Murine) (1)
Conjugates HRP (1241), FITC (1158), Biotin (986), Alkaline Phosphatase (AP) (859), Texas Red (TR) (568), TRITC (375), Rhodamine (369), DyLight 594 (198), DyLight 800 (186), PE (142), DyLight 650 (116), DyLight 633 (115), DyLight 550 (113), DyLight 350 (96), RPE (64), Cy3 (39), Cy5 (39), Gold (37), APC (34), Alexa Fluor 647 (34), Alexa Fluor 350 (33), Alexa Fluor 488 (33), Alexa Fluor 555 (33), Cy5.5 (33), Cy7 (33), PE-Cy3 (33), PE-Cy5 (33), PE-Cy5.5 (33), PE-Cy7 (33), Agarose Beads (27), DyLight 488 (20), SureLight® R-PE (15), DyLight 405 (13), DyLight 680 (13), Atto 425 (10), Atto 488 (10), Atto 532 (10), Atto 550 (10), Atto 594 (10), Atto 647N (10), Atto 655 (10), Colloidal Gold (10nm) (8), Colloidal Gold (5nm) (7), SPRD (7), Beads (6), Peroxidase (POD) (6), Poly-HRP40 (4), beta-Gal (4), Poly-HRP (3), PromoFluor-350 (3), PromoFluor-405 (3), PromoFluor-415 (3), PromoFluor-488 Premium (3), PromoFluor-488LSS (3), PromoFluor-500LSS (3), PromoFluor-505 (3), PromoFluor-510LSS (3), PromoFluor-514LSS (3), PromoFluor-520LSS (3), PromoFluor-532 (3), PromoFluor-546 (3), PromoFluor-555 (3), PromoFluor-590 (3), PromoFluor-610 (3), PromoFluor-633 (3), PromoFluor-647 (3), PromoFluor-670 (3), PromoFluor-680 (3), PromoFluor-700 (3), PromoFluor-750 (3), PromoFluor-770 (3), PromoFluor-780 (3), PromoFluor-840 (3), AMCA (2), Chromeo™ 488 (2), Chromeo™ 546 (2), Colloidal Gold (20nm) (2), SureLight® P3 (2), Chromeo™ 494 (1), Chromeo™ 642 (1), Colloidal Gold (15nm) (1), DTAF (1), FL (1), PE,Cy7 (1), PE,RPE (1), Poly-HRP80 (1)
Epitopes Heavy & Light Chain (3886), Fc Region (821), F(ab')2 Region (697), Fc Region, Chain gamma (78), Chain gamma (66), Heavy Chain (54), H&L (45), Fab Region (43), Chain gamma 1,Chain gamma 2b,Chain gamma 2a,Chain gamma 3 (14), Light Chain (12), Chain gamma,Fc Region (4), Fd Region (4), heavy chain (3), Fc Region,Heavy Chain (2), N-Term (2), Native Conformation (2), Chain alpha (1), Heavy Chain, Fc Region (1)