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Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594)
Heavy & Light Chain
Alternatives DyLight 594
Flow Cytometry (FACS), Immunofluorescence (IF)
|2 references available|
|Quantity||1 mg (1 mg/ml (E 1% at 280 nm = 13.0)) (Variants)|
|Price||171.29 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified Goat IgG, whole molecule|
|Description||Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight 594 Conjugate, min x w/human, mouse, rabbit or rat IgG. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm). Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.|
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
|Application Notes||This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications|
|Concentration||1 mg/ml (E 1% at 280 nm = 13.0)|
|Purity||Affinity purified antibody is > 95% based on SDS-PAGE|
|Purification||Affinity purified using solid phase mouse IgG (H&L)|
|Buffer||10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide|
|Storage||Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.|
|Research Area||Secondary Antibodies|
|Restrictions||For Research Use only|
Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).
Martinez-Exposito, Kaplan, Copeland et al.: "Retention of the BUB3 checkpoint protein on lagging chromosomes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8493-8, 1999 (PubMed).