Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (DyLight 650)
-
- Target See all Ig products
- Ig
-
Binding Specificity
- Chain kappa, Light Chain
-
Reactivity
- Human
-
Host
- Goat
-
Conjugate
- DyLight 650
-
Application
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)
- Specificity
- Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins
- Cross-Reactivity (Details)
- Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins
- Characteristics
-
Concentration: 1.0 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: DyLight 650 (Ex = 652 nm, Em = 672 nm) - Purification
- Affinity purified using solid phase human Kappa (κ) Chain
- Purity
- > 95 % based on SDS-PAGE
- Immunogen
- Purified human kappa (kappa) light chain
-
-
- Application Notes
- This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
- Comment
-
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
- Concentration
- 1.0 mg/mL
- Buffer
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store freeze-dried powder at 2-8 °C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
-
- Target
- Ig
- Alternative Name
- Immunoglobulin Ig (Ig Products)
-