GRB2 antibody (C-Term)

Catalog No. ABIN615588

The Rabbit Polyclonal anti-GRB2 antibody (ABIN615588) specifically detects GRB2 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for GRB2 antibody (C-Term) (ABIN615588)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRB2 Antibody

Binding Specificity: AA 198-217, C-Term

Specificity: The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa adaptor protein.

Purification: Immunoaffinity Chromatography

Immunogen: GRB2 antibody was raised against synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217). .Remarks: 100% identity between man, cow, rat and mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 15 mM sodium azide and 0.2 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2 / ASH

Background: Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2

Gene ID: 2885

NCBI Accession: NP_002077

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation