IL-1 beta antibody

Catalog No. ABIN616079

The Rabbit Polyclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Mouse and Rat. It has been validated for WB, IP, IF, IHC (p), Neut, EIA, IHC (fro) and RIA.

Quick Overview for IL-1 beta antibody (ABIN616079)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-IL-1 beta Antibody

Specificity: This antibody is primarily directed against mature, 17,000 MW Mouse IL1-beta and is useful in determining its presence in various assays. The antibody does not recognize Human IL1-beta or Mouse IL1-alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with Rat IL1-beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW Mouse IL1-beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in Immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1-beta precursor molecule. Denatured 31,000 precursor IL1-beta will be recognized by this antibody.

Purification: DEAE Fractionation

Immunogen: Recombinant Mouse IL-1 beta produced in E. coli Genename: IL1b Remarks: The MW of the recombinant Mouse IL-1 beta is 17 kDa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent)

Concentration: 1.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, None, None

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C or -20 °C. Following reconstitution store at -20 °C.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta / IL-1B

Background: IL-1 beta (also known as Interleukin-1 beta, IL1-beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gene ID: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome