CD3 (CD3) antibody

Details for Product No. ABIN616760
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Antigen
Synonyms CD3
Reactivity
Human, Monkey
(480), (135), (59), (28), (24), (16), (12), (7), (6), (5), (5), (3), (2)
Host
Mouse
(508), (88), (72), (46)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(118), (74), (73), (27), (7), (7), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
(523), (106), (105), (100), (92), (92), (65), (48), (47), (27), (23), (10), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 12 references available
Quantity 0.1 mg
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Catalog No. ABIN616760
308.00 $
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Immunogen Human thymocytes followed by Sezary T cells.
Clone UCHT1
Isotype IgG1
Specificity This antibody recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
Cross-Reactivity (Details) Species reactivity (tested):Human, Non-Human Primates.
Purification Protein-A Affinity Chromatography
Alternative Name CD3
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Azide Free phosphate buffered saline (PBS), pH ~7.4 and 0.2 μm filter sterilized
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Fisch, Malkovsky, Braakman et al.: "Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis." in: The Journal of experimental medicine, Vol. 171, Issue 5, pp. 1567-79, 1990 (PubMed).

Garson, Beverley, Coakham et al.: "Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species." in: Nature, Vol. 298, Issue 5872, pp. 375-7, 1982 (PubMed).

le Gouvello, Manceau, Sobel: "Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1113-22, 1998 (PubMed).

Torres, Alcover, Zapata et al.: "TCR dynamics in human mature T lymphocytes lacking CD3 gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5947-55, 2003 (PubMed).

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004 (PubMed).

Arnett, Harrison, Wiley: "Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 46, pp. 16268-73, 2004 (PubMed).

Demedts, Brusselle, Vermaelen et al.: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006 (PubMed).

Alarcón, Swamy, van Santen et al.: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006 (PubMed).

Rieux-Laucat, Hivroz, Lim et al.: "Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency." in: The New England journal of medicine, Vol. 354, Issue 18, pp. 1913-21, 2006 (PubMed).

Siegers, Swamy, Fernández-Malavé et al.: "Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2537-44, 2007 (PubMed).

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