Chicken anti-Goat IgG (Heavy & Light Chain) Antibody (TRITC)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Goat
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Host
- Chicken
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.
- Cross-Reactivity (Details)
- Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.
- Characteristics
- Chicken anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase goat IgG.
- Purification
- Affinity purification
- Isotype
- IgY
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- Application Notes
- Needs to be determined by end user for immunocytochemistry (ICC), immunohistochemistry (IHC) and for flow cytometry.
- Comment
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BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Shelf life of this product is one year from date of receipt.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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