Goat anti-Rat IgG (Heavy & Light Chain) Antibody (HRP)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- Immunoassay (IA)
- Characteristics
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Goat anti-rat IgG (H&L), HRP conjugated, is a F(ab) '2 fragment of a secondary antibody which binds to rat IgGs in immunological assasys. Antibodies are adsorbed against human, mouse IgG and affinity purified. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment - Purification
- Affinity purification
- Purity
- > 90 % based on SDS-PAGE.
- Isotype
- IgG
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- Application Notes
- Needs to be determined by end user for all immunoassay applications.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Handling Advice
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - Storage
- 4 °C
- Storage Comment
- Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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