Chicken anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN619641

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Chicken

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine)

Predicted Reactivity: rabbit IgG (H&L)

Characteristics: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

Purification: Affinity purification

Immunogen: Purified Rabbit IgG

Isotype: IgY

Application Details

Application Notes: optimal working dilution should be determined by the investigator

Comment:

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody