MICA antibody (Middle Region)

Catalog No. ABIN630278

The Rabbit Polyclonal anti-MICA antibody is suitable to detect MICA in samples from Human. It has been validated for WB.

Quick Overview for MICA antibody (Middle Region) (ABIN630278)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MICA Antibody

Binding Specificity: Middle Region

Specificity: MICA antibody was raised against the middle region of MICA

Purification: Purified

Immunogen: MICA antibody was raised using the middle region of MICA corresponding to a region with amino acids LRRTVPPMVNVTRSEASEGNITVTCRASGFYPWNITLSWRQDGVSLSHDT

Application Details

Application Notes: WB: 2.5 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

MICA Blocking Peptide, (ABIN5614764), is also available for use as a blocking control in assays to test for specificity of this MICA antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MICA antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA

Background: MICA is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognised by intestinal epithelial gamma delta T cells.MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognised by intestinal epithelial gamma delta T cells.

Molecular Weight: 42 kDa (MW of target protein)

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response