anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (Dimer gamma-epsilon)

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Antigen
Synonyms cd3e, CD3E, t3e, tcre, AI504783, CD3, CD3epsilon, T3e, T3E, TCRE
Alternatives
Epitope
Dimer gamma-epsilon, Dimer delta-epsilon
(31), (15), (13), (13), (13), (11), (10), (7), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(467), (239), (59), (43), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Host
Mouse
(220), (160), (151), (88), (69), (24), (13), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3E antibody is un-conjugated
(97), (75), (69), (46), (17), (17), (15), (9), (9), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
(539), (181), (178), (139), (65), (62), (52), (49), (34), (22), (17), (12), (9), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Quantity 0.1 mg
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Immunogen Human thymocytes and T lymphocytes.
Clone MEM-57
Isotype IgG2a
Specificity The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. HLDA IV., WS Code T 96
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-82 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling
Application Notes Flow Cytometry: Recommended dilution: 2 - 5 µg/mL
Positive control: Peripheral Blood LymphocytesJURKAT human leukemia T cell line
Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7).
Functional Application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Majer, Vlaskova, Krol et al.: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Panyi, Bagdány, Bodnár et al.: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003 (PubMed).

Dave, Cao, Browne et al.: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997 (PubMed).

Hilgert, Fran?k, Stefanová et al.: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993 (PubMed).

Soucek, Hilgert, Budová et al.: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994 (PubMed).

Soucek, Chudomel, Hrubá et al.: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, 1991 (PubMed).

Background publications Alarcón, Swamy, van Santen et al.: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006 (PubMed).

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006 (PubMed).

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004 (PubMed).

Horejsí, Angelisová, Bazil et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988 (PubMed).

Catalog No. ABIN638428
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