WD Repeat Domain Containing 82 (WDR82) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN651007
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Antigen
Synonyms MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WDR82A
Epitope
N-Term, AA 1-30
(1), (1)
Reactivity
Human
(1)
Host
Rabbit
(1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(1), (1), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN651007
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Immunogen This WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human WDR82.
Clone RB25812
Isotype Ig
Specificity This WDR82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human WDR82.
Predicted Reactivity Chicken,Mouse (Murine),Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name WDR82
Background WDR82 is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes.
Synonyms: Swd2, WD repeat-containing protein 82,UNQ9342/PRO34047, WDR82A, WDR82
Molecular Weight 35079 DA
Gene ID 80335
UniProt Q6UXN9
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-WD Repeat Domain Containing 82 (WDR82) (N-Term), (AA 1-30) antibody Western blot analysis of WDR82 Antibody (N-term) (ABIN651007) in HepG2 cell line lysates (35 µg/lane). WDR82 (arrow) was detected using the purified polyclonal antibody.
anti-WD Repeat Domain Containing 82 (WDR82) (N-Term), (AA 1-30) antibody (2) WDR82 Antibody (N-term) (ABIN651007) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-WD Repeat Domain Containing 82 (WDR82) (N-Term), (AA 1-30) antibody (3) WDR82 Antibody (N-term) (ABIN651007) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Higa, Wu, Ye et al.: "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation." in: Nature cell biology, Vol. 8, Issue 11, pp. 1277-83, 2006 (PubMed).

Lee, Skalnik: "Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 609-18, 2008 (PubMed).

Wu, Wang, Lee et al.: "Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS." in: Molecular and cellular biology, Vol. 28, Issue 24, pp. 7337-44, 2008 (PubMed).

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