Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 149-179)

Details for Product anti-CASP3 Antibody No. ABIN651176, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Alternatives
anti-Human Caspase 3 antibody for BioImaging
Epitope
AA 149-179, Asp175
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32
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Reactivity
Human
560
306
255
48
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24
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12
9
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2
2
Host
Rabbit
456
158
18
4
2
Clonality (Clone)
Polyclonal ()
Conjugate
This Caspase 3 antibody is un-conjugated
27
24
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14
9
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4
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Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
477
238
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141
107
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55
50
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29
28
13
9
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2
2
2
2
2
2
2
1
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Options
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Immunogen This CASP3(Asp175) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-179 AA from human CASP3(Asp175).
Clone RB25834
Isotype Ig
Specificity This CASP3(Asp175) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 155-185 from the Asp175 region of human CASP3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CASP3 (CASP3 Antibody Abstract)
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Synonyms: Caspase-3 subunit p12, Caspase-3 subunit p17, SREBP cleavage activity 1, SCA-1, Protein Yama, Cysteine protease CPP32, CPP-32, Apopain, Caspase-3, CASP-3,CPP32, CASP3
Molecular Weight 31608 DA
Gene ID 836
NCBI Accession NP_004337
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.33 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 149-179) (ABIN651176) Western blot analysis of CASP3 (Asp175) Antibody (ABIN651176) in CEM cell line lysate...
Immunohistochemistry (IHC) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 149-179) (ABIN651176) CASP3(Asp175) Antibody (ABIN651176) IHC analysis in formalin fixed and paraffin embed...
Western Blotting (WB) image for anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 149-179) (ABIN651176) Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (ABIN65...
Background publications Mei, Yong, Liu, Shi, Meinkoth, Dreyfuss, Yang: "tRNA binds to cytochrome c and inhibits caspase activation." in: Molecular cell, Vol. 37, Issue 5, pp. 668-78, 2010 (PubMed).