Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) (Center) antibody

Antigen: Insulin-Like Growth Factor Binding Protein 4 (IGFBP4)

Synonyms: BP-4, IBP4, IGFBP-4, HT29-IGFBP, Deb2, AI875747, IGFBP4, IGF-BP4, igfbp4

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652262

Additional Information

Alternative name: IGFBP4

Gene ID: 3487

UniProt: P22692, NP_001543.2

Immunogen: This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human IGFBP4.

Isotype: Ig

Description: Other names: Insulin-like growth factor-binding protein 4,IGFBP4,IBP4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human IGFBP4.

Molecular Weight: 27934 DA

Comments:

Background: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

IGFBP4 Antibody (Center) (ABIN652262) western blot analysis in mouse lung tissue lysates (15ug/lane).This demonstrates the IGFBP4 antibody detected IGFBP4 protein (arrow).

IGFBP4 antibody (Center) (ABIN652262) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGFBP4 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Canzian, Cox, Setiawan et al.: "Comprehensive analysis of common genetic variation in 61 genes related to steroid hormone and insulin-like growth factor-I metabolism and breast cancer risk in the NCI breast and prostate cancer cohort consortium." in: Human molecular genetics, Vol. 19, Issue 19, pp. 3873-84, 2010 (PubMed).