HIF1A antibody (AA 519-547)

Catalog No. ABIN652389

Cited in 2+ publications. The Rabbit Polyclonal anti-HIF1A antibody (Clone RB14548) (ABIN652389) specifically detects HIF1A in WB, IF, FACS and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for HIF1A antibody (AA 519-547) (ABIN652389)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB14548

Product Details anti-HIF1A Antibody

Binding Specificity: AA 519-547

Predicted Reactivity: B, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:250. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-HIF1A antibody (ABIN652389)

Li, Qu, Jin, Guo, Wang, Qi, Yang, Zhang, Li: "Protective effect of salidroside against bone loss via hypoxia-inducible factor-1α pathway-induced angiogenesis." in: Scientific reports, Vol. 6, pp. 32131, (2018) (PubMed).

Wang, Huang, Guan, Xiao, Deng, Chen, Chen, Li, Huang, Shi: "Hypoxia-inducible factor-1alpha and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation." in: PLoS ONE, Vol. 8, Issue 9, pp. e74051, (2013) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1Alpha

Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.

Molecular Weight: 92670

Gene ID: 3091

NCBI Accession: NP_001230013, NP_001521, NP_851397

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect