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phosphoprotein Enriched in Astrocytes 15 (PEA15) (AA 86-115), (C-Term) antibody

Details for Product No. ABIN652463, Supplier: Log in to see
Antigen
  • PEA15
  • DKFZp459O1410
  • HMAT1
  • HUMMAT1H
  • MAT1
  • MAT1H
  • PEA-15
  • PED
  • Pea15a
  • Mat1
  • Pea15
  • Pkcs15
Epitope
AA 86-115, C-Term
29
16
10
5
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
101
61
53
7
1
1
Host
Rabbit
98
3
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
66
57
40
17
15
12
4
2
1
1
1
Supplier
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Immunogen This PEA-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 AA from the C-terminal region of human PEA-15.
Clone RB21195
Isotype Ig
Specificity This PEA-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the C-terminal region of human PEA-15.
Predicted Reactivity Hamster,Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PEA-15 (PEA15 Antibody Abstract)
Background PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.
Synonyms: Astrocytic phosphoprotein PEA-15, Phosphoprotein enriched in diabetes, PED, PEA15
Molecular Weight 15040 DA
Gene ID 8682
UniProt Q15121
Research Area Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (AA 86-115), (C-Term) antibody (ABIN652463) Western blot analysis of PEA-15 Antibody (C-term) (ABIN652463) in T47D cell line lysa...
Western Blotting (WB) image for anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (AA 86-115), (C-Term) antibody (ABIN652463) Western blot analysis of PEA-15 Antibody (C-term) (ABIN652463) in mouse lung tissue l...
Immunohistochemistry (IHC) image for anti-phosphoprotein Enriched in Astrocytes 15 (PEA15) (AA 86-115), (C-Term) antibody (ABIN652463) Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody ...
Product cited in: Lv, Ma, Zhang et al.: "Quantitative proteomics reveals that PEA15 regulates astroglial A? phagocytosis in an Alzheimer's disease mouse model." in: Journal of proteomics, Vol. 110C, pp. 45-58, 2014 (PubMed).

Background publications Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Trencia, Perfetti, Cassese et al.: "Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4511-21, 2003 (PubMed).