BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) (Center) antibody

Antigen: BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)

Synonyms: BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652493

Additional Information

Alternative name: BCL2L11

Gene ID: 10018

UniProt: O43521

Immunogen: This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the Center region of human BCL2L11.

Isotype: Ig

Description: Other names: Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BIM, BCL2L11

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the Center region of human BCL2L11.

Molecular Weight: 22171 DA

Comments:

Background: BCL2L11 belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of BCL2L11 Antibody (Center) (ABIN652493) in K562, HL-60 cell line lysates (35ug/lane). BCL2L11 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with BCL2L11 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of WiDr cells using BCL2L11 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Putcha, Le, Frank et al.: "JNK-mediated BIM phosphorylation potentiates BAX-dependent apoptosis." in: Neuron, Vol. 38, Issue 6, pp. 899-914, 2003 (PubMed).

Hippe, Weber, Zhou et al.: "Toxoplasma gondii infection confers resistance against BimS-induced apoptosis by preventing the activation and mitochondrial targeting of pro-apoptotic Bax." in: Journal of cell science, Vol. 122, Issue Pt 19, pp. 3511-21, 2009 (PubMed).