Retinoblastoma 1 (RB1) (C-Term) antibody

Antigen: Retinoblastoma 1 (RB1)

Synonyms: RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652516

Additional Information

Alternative name: RB1

Gene ID: 5925

UniProt: P06400

Immunogen: This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1

Isotype: Ig

Description: Other names: Retinoblastoma-associated protein, pRb, Rb, pp110, p105-Rb, RB1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1

Molecular Weight: 106159 DA

Comments:

Background: RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RB1 Antibody (C-term) (ABIN652516) in 293 cell line lysates (35ug/lane). RB1 (arrow) was detected using the purified Pab.(2ug/ml)

Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

RB1 Antibody (C-term) (ABIN652516) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).

Connell-Crowley, Harper, Goodrich: "Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 287-301, 1997 (PubMed).