|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Retinoblastoma 1 (RB1) (C-Term) antibody
|Synonyms||RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA
|2 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1|
|Description||Other names: Retinoblastoma-associated protein, pRb, Rb, pp110, p105-Rb, RB1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 865~894 amino acids from the C-terminal region of human RB1|
|Molecular Weight||106159 DA|
Background: RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Transcription Factors, Signaling, Neurology, Cell Structure|
|Restrictions||For Research Use only|
Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).
Connell-Crowley, Harper, Goodrich: "Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 287-301, 1997 (PubMed).