Suppressor of Cytokine Signaling 1 (SOCS1) (N-Term) antibody

Antigen: Suppressor of Cytokine Signaling 1 (SOCS1)

Synonyms: JAB, CIS1, SSI1, TIP3, CISH1, SSI-1, SOCS-1, Cish1, Socs-1, zgc:91868, SOCS1, jab, cis1, ssi1, tip3, cish1, ssi-1, socs-1, socs1, LOC100228306, socs1b

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652738

Additional Information

Alternative name: SOCS1

Gene ID: 8651

UniProt: O15524

Immunogen: This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43~73 amino acids from the N-terminal region of human SOCS1.

Isotype: Ig

Description: Other names: Tec-interacting protein 3, TIP-3, STAT-induced STAT inhibitor 1, SSI-1, JAK-binding protein, JAB, Suppressor of cytokine signaling 1, SOCS-1, SOCS1, SSI1, TIP3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43~73 amino acids from the N-terminal region of human SOCS1.

Molecular Weight: 23551 DA

Comments:

Background: SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of SOCS1 Antibody (N-term) (ABIN652738) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) (ABIN652738) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Publications

Product: Starr, Willson, Viney et al.: "A family of cytokine-inducible inhibitors of signalling." in: Nature, Vol. 387, Issue 6636, pp. 917-21, 1997 (PubMed).

Minamoto, Ikegame, Ueno et al.: "Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3." in: Biochemical and biophysical research communications, Vol. 237, Issue 1, pp. 79-83, 1997 (PubMed).