Unc-13 Homolog B (C. Elegans) (UNC13B) (Center) antibody

Antigen: Unc-13 Homolog B (C. Elegans) (UNC13B)

Synonyms: UNC13, MUNC13, Unc13h2, hmunc13, MGC133279, MGC133280, Munc13-2, UNC13B, DKFZp469L231, Unc13a, Unc13h1, Munc13-1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652852

Additional Information

Alternative name: UNC13B

Gene ID: 10497

UniProt: O14795

Immunogen: This UNC13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1069~1099 amino acids from the Center region of human UNC13B.

Isotype: Ig

Description: Other names: Munc13-2, munc13, Protein unc-13 homolog B, UNC13B, UNC13

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UNC13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1069~1099 amino acids from the Center region of human UNC13B.

Molecular Weight: 180679 DA

Comments:

Background: UNC13B plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. It is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UNC13B Antibody (Center) (ABIN652852) in 293 cell line lysates (35ug/lane). UNC13B (arrow) was detected using the purified Pab.

UNC13B Antibody (Center) (ABIN652852) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).