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Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody

Details for Product No. ABIN652928, Supplier: Log in to see
Antigen
  • rama1
  • C13orf3
  • RAMA1
  • F630043A04Rik
  • RGD1307201
  • C12H13ORF3
  • fc23d11
  • si:rp71-68n21.13
  • wu:fc23d11
Epitope
AA 226-253, Center
21
10
9
2
1
1
1
Reactivity
Human
42
12
12
4
Host
Rabbit
35
7
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
34
30
9
5
2
2
2
2
Supplier
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Immunogen This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 AA from the Central region of human C13orf3.
Clone RB23058
Isotype Ig
Specificity This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name C13orf3 (SKA3 Antibody Abstract)
Background Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
Synonyms: Spindle and kinetochore-associated protein 3, SKA3, C13orf3, RAMA1
Molecular Weight 46359 DA
Gene ID 221150
UniProt Q8IX90
Research Area Cancer, Cell Cycle, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.36 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody (ABIN652928) Western blot analysis of C13orf3 Antibody (Center) (ABIN652928) in Hela, 293 cell lin...
Immunohistochemistry (IHC) image for anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody (ABIN652928) C13orf3 Antibody (Center) (ABIN652928) IHC analysis in formalin fixed and paraffin em...
Flow Cytometry (FACS) image for anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253), (Center) antibody (ABIN652928) C13orf3 Antibody (Center) (ABIN652928) flow cytometric analysis of Hela cells (right ...
Background publications Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).