Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (Center) antibody

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14)

Synonyms: TR2, ATAR, HVEA, HVEM, LIGHTR, MGC108903, TNFRSF14

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653038

Additional Information

Alternative name: TNFRSF14

Gene ID: 8764

UniProt: Q92956

Immunogen: This TNFRSF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human TNFRSF14.

Isotype: Ig

Description: Other names: Tumor necrosis factor receptor-like 2, TR2, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, Tumor necrosis factor receptor superfamily member 14, UNQ329/PRO509, HVEM, HVEA, TNFRSF14

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TNFRSF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human TNFRSF14.

Molecular Weight: 30392 DA

Comments:

Background: TNFRSF14 is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of anti-TNFRSF14 Antibody (Center) Pab (ABIN653038) pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. TNFRSF14 (arrow) was detected using the purified Pab (1:250 dilution).

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TNFRSF14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

TNFRSF14 Antibody (Center) (ABIN653038) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dubois, Trynka, Franke et al.: "Multiple common variants for celiac disease influencing immune gene expression." in: Nature genetics, Vol. 42, Issue 4, pp. 295-302, 2010 (PubMed).