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A1CF antibody (APOBEC1 Complementation Factor) (AA 394-423)

Details for Product anti-A1CF Antibody No. ABIN654627, Supplier: Log in to see
Antigen
  • 1810073H04Rik
  • A1CF
  • A1cft
  • acf
  • Acf
  • ACF
  • acf64
  • ACF64
  • ACF65
  • acf65
  • Apobec-1
  • apobec1cf
  • APOBEC1CF
  • asp
  • ASP
Epitope
AA 394-423, C-Term
17
16
7
3
3
2
1
1
Reactivity
Human
42
5
4
1
1
1
1
Host
Rabbit
38
4
Clonality (Clone)
Polyclonal ()
Conjugate
This A1CF antibody is un-conjugated
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
38
23
18
14
6
4
1
1
1
1
Supplier
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Immunogen This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 AA from the C-terminal region of human ACF.
Clone RB20614
Isotype Ig
Specificity This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-427 amino acids from the C-terminal region of human ACF.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ACF (A1CF Antibody Abstract)
Background Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.
Synonyms: APOBEC1 complementation factor,A1CF,ACF, ASP
Molecular Weight 65202 DA
Gene ID 29974
NCBI Accession NP_620310
UniProt Q9NQ94
Research Area Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Flow Cytometry (FACS) image for anti-A1CF antibody (APOBEC1 Complementation Factor) (AA 394-423) (ABIN654627) ACF Antibody (C-term) (ABIN654627) flow cytometric analysis of A375 cells (right hist...
Background publications Galloway, Smith: "The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 659-63, 2010

Blanc, Henderson, Newberry, Kennedy, Luo, Davidson: "Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality." in: Molecular and cellular biology, Vol. 25, Issue 16, pp. 7260-9, 2005

Xie, Sowden, Dance, Torelli, Smith, Wedekind: "The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8114-9, 2004

Deloukas, Earthrowl, Grafham, Rubenfield, French, Steward, Sims, Jones, Searle, Scott, Howe, Hunt, Andrews, Gilbert, Swarbreck, Ashurst, Taylor, Battles, Bird, Ainscough, Almeida, Ashwell, Ambrose et al.: "The DNA sequence and comparative analysis of human chromosome 10. ..." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004

Blanc, Kennedy, Davidson: "A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41198-204, 2003