NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, Assembly Factor 1 (NDUFAF1) (C-Term) antibody

Antigen: NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, Assembly Factor 1 (NDUFAF1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654702

Additional Information

Alternative name: NDUFAF1

Gene ID: 51103

UniProt: Q9Y375, NP_057097.2

Immunogen: This NDUFAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-280 amino acids from the C-terminal region of human NDUFAF1.

Isotype: Ig

Description: Other names: Complex I intermediate-associated protein 30, mitochondrial,NDUFAF1,CIA30

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NDUFAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-280 amino acids from the C-terminal region of human NDUFAF1.

Molecular Weight: 37764 DA

Comments:

Background: The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

NDUFAF1 antibody (C-term) (ABIN654702) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFAF1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Dunning, McKenzie, Sugiana et al.: "Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease." in: The EMBO journal, Vol. 26, Issue 13, pp. 3227-37, 2007 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).