Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (C-Term), (AA 219-248) antibody

Details for Product No. ABIN655271
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Antigen
Synonyms
CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRICE, Dmel\\CG7788, DrICE, DrIce, Dric ... show more
CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRICE, Dmel\\CG7788, DrICE, DrIce, Drice, ICE, crice, drICE, drIce, drice, ice, A830040C14Rik, AC-3, Apopain, CPP32, Lice, Yama, mldy, CPP32B, SCA-1, xcpp32 show less
Epitope
C-Term, AA 219-248
(28), (21), (21), (15), (14), (12), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(308), (118), (94), (19), (18), (18), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(275), (60), (7), (2), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(10), (8), (6), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(263), (118), (99), (91), (54), (31), (26), (19), (19), (14), (10), (4), (2), (2), (2)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN655271
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Immunogen This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 AA from the C-terminal region of human CASP3.
Clone RB14883
Isotype Ig
Specificity This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human CASP3.
Predicted Reactivity Mouse (Murine),Monkey,Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CASP3
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
Synonyms: Caspase-3,CASP3,CPP32
Molecular Weight 31608 DA
Gene ID 836
NCBI Accession NP_004337, NP_116786
UniProt P42574
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (C-Term), (AA 219-248) antibody CASP3 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (C-Term), (AA 219-248) antibody (2) CASP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CASP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (C-Term), (AA 219-248) antibody (3) CASP3 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Burke, Smith, Smith: "cIAP1 cooperatively inhibits procaspase-3 activation by the caspase-9 apoptosome." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30061-8, 2010 (PubMed).

Würstle, Laussmann, Rehm: "The caspase-8 dimerization/dissociation balance is a highly potent regulator of caspase-8, -3, -6 signaling." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33209-18, 2010 (PubMed).

Srikanth, Wall, Maldonado-Contreras et al.: "Salmonella pathogenesis and processing of secreted effectors by caspase-3." in: Science (New York, N.Y.), Vol. 330, Issue 6002, pp. 390-3, 2010 (PubMed).

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