Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Okumura, Minamiyama, Ho, Kay, Kawasaki, Tourtas, Schlötzer-Schrehardt, Kruse, Young, Quantock, Kinoshita, Koizumi: "Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in Fuchs endothelial corneal dystrophy." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 11, pp. 1291-304, (2015) (PubMed).
Target
COL8a2 (COL8A2)
(Collagen, Type VIII, alpha 2 (COL8A2))
FECD antibody, FECD1 antibody, PPCD antibody, PPCD2 antibody, AI429819 antibody, collagen type VIII alpha 2 chain antibody, collagen, type VIII, alpha 2 antibody, COL8A2 antibody, Col8a2 antibody
Background
This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.