HIST1H2BC/HIST1H2BF (N-Term), (AA 1-30) antibody

Details for Product No. ABIN656773
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Antigen
Epitope
N-Term, AA 1-30
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN656773
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Immunogen This HIST1H2BC/HIST1H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human HIST1H2BC/HIST1H2BF.
Clone RB32591
Isotype Ig
Specificity This HIST1H2BC/HIST1H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BC/HIST1H2BF.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Monkey,Xenopus laevis,Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: Histone H2B type 1-C/E/F/G/I,HIST1H2BC,H2BFL
Molecular Weight 13906 DA
Gene ID 8347
NCBI Accession NP_003509, NP_003514, NP_003513, NP_003516, NP_003517
UniProt P62807
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment HIST1H2BC/HIST1H2BF Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.Precau.
Expiry Date 6 months
Supplier Images
anti-HIST1H2BC/HIST1H2BF (N-Term), (AA 1-30) antibody HIST1H2BC/HIST1H2BF Antibody (N-term) (ABIN656773) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane). This demonstrates the HIST1H2BC/HIST1H2BF antibody detected the HIST1H2BC/HIST1H2BF protein (arrow).
Background publications Kim, Sprung, Chen et al.: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).

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