Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH) (Center) antibody

Antigen: Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse) (ITCH)

Synonyms: AIF4, AIP4, NAPP1, dJ468O1.1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657549

Additional Information

Alternative name: ITCH

Gene ID: 83737

UniProt: Q96J02, NP_113671.3

Immunogen: This ITCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-536 amino acids from the Central region of human ITCH.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase Itchy homolog,ITCH,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ITCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-536 amino acids from the Central region of human ITCH.

Molecular Weight: 102803 DA

Comments:

Background: Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: ITCH Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

ITCH Antibody (Center) (ABIN657549) western blot analysis in mouse brain tissue lysates (35ug/lane). This demonstrates the ITCH antibody detected the ITCH protein (arrow).

Publications

Product: Lohr, Molleston, Strauss et al.: "Human ITCH E3 ubiquitin ligase deficiency causes syndromic multisystem autoimmune disease." in: American journal of human genetics, Vol. 86, Issue 3, pp. 447-53, 2010 (PubMed).