NCK Adaptor Protein 1 (NCK1) (N-Term), (AA 63-92) antibody

Details for Product No. ABIN657648
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Antigen
Synonyms NCK, NCKalpha, nck-1, NCK1
Epitope
N-Term, AA 63-92
(4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(44), (13), (12), (4)
Host
Rabbit
(27), (18)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(43), (17), (14), (7), (7), (5), (5), (3), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN657648
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 AA from the N-terminal region of human NCK1.
Clone RB33697
Isotype Ig
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NCK1
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Synonyms: Cytoplasmic protein NCK1,NCK1,NCK
Molecular Weight 42864 DA
Gene ID 4690
UniProt P16333, NP_006144.1, NP_001177725.1
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NCK1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
anti-NCK Adaptor Protein 1 (NCK1) (N-Term), (AA 63-92) antibody Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) (ABIN657648). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.
Background publications Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010 (PubMed).

Gehmlich, Hayess, Legler et al.: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010 (PubMed).

Barda-Saad, Shirasu, Pauker et al.: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010 (PubMed).

Hosts (27), (18)
Reactivities (44), (13), (12), (4)
Applications (43), (17), (14), (7), (7), (5), (5), (3), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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