HLA-E antibody (AA 251-358) (HRP)

Catalog No. ABIN674343

The HRP-conjugated Rabbit Polyclonal anti-HLA-E antibody (ABIN674343) specifically detects HLA-E in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for HLA-E antibody (AA 251-358) (HRP) (ABIN674343)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-E antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HLA-E Antibody (HRP)

Binding Specificity: AA 251-358

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HLA E

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: HLA E

Background:

Synonyms: MHC, QA1, EA1.2, EA2.1, HLA-6.2, HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E, HLA-E, HLAE

Background: HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Gene ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response