Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 196-210), (pThr202), (pTyr204) antibody

Details for Product No. ABIN682933, Supplier: Log in to see
Antigen
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
Epitope
pThr202, pTyr204, AA 196-210
128
126
35
34
30
19
18
15
15
15
15
14
12
9
9
9
9
8
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
421
320
304
56
23
15
9
8
8
7
7
6
5
5
3
3
2
2
2
1
1
1
1
Host
Rabbit
349
78
7
Clonality
Polyclonal
Conjugate
Un-conjugated
12
11
11
10
7
7
7
7
6
6
6
6
6
6
2
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
305
114
83
77
59
56
46
42
16
10
6
4
4
4
2
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen KLH conjugated synthetic phosphopeptide derived from human p44/42 MAPK around the phosphorylation site of (Thr202/Tyr204)
Isotype IgG
Purification Purified by Protein A.
Alternative Name Erk1/2 (MAPK1/3 Antibody Abstract)
Sub Type Cocktail
Background

p44/42 MAP Kinase(Thr202), ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Subcellular location: Cytoplasm, Nucleus

Synonyms: ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, MAPK3

Gene ID 5595
UniProt P27361
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 196-210), (pThr202), (pTyr204) antibody (ABIN682933) Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1/2(Thr202 + Tyr204...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 196-210), (pThr202), (pTyr204) antibody (ABIN682933) Formalin-fixed and paraffin embedded human liver labeled with Anti-phospho-ERK1/2(Thr...
Immunofluorescence (IF) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 196-210), (pThr202), (pTyr204) antibody (ABIN682933) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-E...
Product cited in: Wang, Chen, Tang, Jiang, Ji: "AMD3100 Attenuates Matrix Metalloprotease-3 and -9 Expressions and Prevents Cartilage Degradation in a Monosodium Iodo-Acetate-Induced Rat Model of Temporomandibular Osteoarthritis." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 74, Issue 5, pp. 927.e1-927.e13, 2016 (PubMed).

Liu, Jiang, Zhang, Li, Li, Xie, Hu: "Pulmonary artery denervation improves pulmonary arterial hypertension induced right ventricular dysfunction by modulating the local renin-angiotensin-aldosterone system." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 192, 2016 (PubMed).

Liu, Huang, Wang, Song, Cen, Ji: "Anthraquinone derivative exerted hormetic effect on the apoptosis in oxygen-glucose deprivation-induced PC12 cells via ERK and Akt activated Nrf2/HO-1 signaling pathway." in: Chemico-biological interactions, Vol. 262, pp. 1-11, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Geng, Zhu, Xie, Li, Wu, Liang, Xie, Zhu, Huang, Zhu, Wu, Zhong: "Medium-chain triglyceride ameliorates insulin resistance and inflammation in high fat diet-induced obese mice." in: European journal of nutrition, 2015 (PubMed).

Chen, Bao, Chen, Burton, Gong, Gu, Mi, Bao: "Mycobacterium tuberculosis PE25/PPE41 protein complex induces activation and maturation of dendritic cells and drives Th2-biased immune responses." in: Medical microbiology and immunology, 2015 (PubMed).

Rosenzweig, Ou, Quinn: "P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture." in: Journal of cellular and molecular medicine, Vol. 17, Issue 4, pp. 508-17, 2013 (PubMed).

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