AKT 1/2/3 antibody (pSer473) (Cy3)

Catalog No. ABIN729301

Product Details anti-AKT 1/2/3 Antibody (Cy3)

Target: AKT 1/2/3

Binding Specificity: pSer473

Reactivity: Human, Mouse, Rat, Cow, Chicken, Rabbit, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Chicken, Cow, Fish, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/2/3 (AKT 1/2/3 Products)

Background:

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, RAC-PK-alpha, Akt1

Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Gene ID: 24185

UniProt: P47196