DYKDDDDK Tag antibody

Details for Product No. ABIN730348, Supplier: Log in to see
Antigen
Reactivity
Tag
237
101
65
18
18
9
8
7
4
3
2
1
1
1
Host
Rabbit
198
108
34
34
17
Clonality
Polyclonal
Conjugate
Un-conjugated
39
21
18
10
10
10
8
8
7
7
7
7
7
7
6
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
312
131
126
103
84
58
43
14
10
8
7
7
4
4
4
4
3
3
3
2
1
1
1
1
1
Options
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Immunogen MAP of Flag Tag peptide
Sequence DYKDDDDK
Isotype IgG
Characteristics Antibody is specific for: FLAG Tag
Purification Purified by Protein A.
Alternative Name FLAG Tag
Target Type Tag
Background

This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.

Synonyms: DDDDK epitope tag, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, flag, DDDDK tag N-T.

Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Product cited in: Zhang, Feng, Li, Wang, Li, Sun, Li, Zheng, Liu, Yang, Niu, Zhong, Chen: "Effects of the fusion design and immunization route on the immunogenicity of Ag85A-Mtb32 in adenoviral vectored tuberculosis vaccine." in: Human vaccines & immunotherapeutics, Vol. 11, Issue 7, pp. 1803-13, 2015 (PubMed).

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