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DYKDDDDK Tag antibody

WB, ELISA, IP, IF Host: Mouse Monoclonal 4G5C8 unconjugated

Immunoprecipitating Flag Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG instead of in Flag Tag in 293F transfected whole cell lysate. (DYKDDDDK Tag antibody)

Western Blot Positive WB detected in: 10 ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa (DYKDDDDK Tag antibody)

Western Blot Positive WB detected in: Flag Tag fusion protein at 50 ng, 25 ng, 12. (DYKDDDDK Tag antibody)

Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate Lane 1: Mouse control IgG instead of in Transfected HEK-293 cell whole cell lysate Lane 2: (1 μL) + Transfected HEK-293 cell whole cell lysate (500 μg) Lane 3: Transfected HEK-293 cell whole cell lysate (20 μg) For western blotting, the blot was detected with at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 (DYKDDDDK Tag antibody)

Western Blot Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50 ng All lanes: Flag Tag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 59. (DYKDDDDK Tag antibody)

Immunofluorescence staining of 293T cells transfected with Flag tag by at 1:300, counter-stained with DAPI. (DYKDDDDK Tag antibody)

6 images

Catalog No. ABIN7322021

Datasheet as PDF

Target See all DYKDDDDK Tag products

DYKDDDDK Tag

Reactivity

Please inquire
Host
57

51

8

6

6
Mouse
Compatible Secondaries
Clonality
64

63
Monoclonal
Conjugate
45

15

10

7

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DYKDDDDK Tag antibody is un-conjugated
Application
78

61

28

23

19

17

12

12

10

6

5

4

3

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Purification

Protein G purified

Immunogen

DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Clone

4G5C8

Isotype

IgG2a
anti-DYKDDDDK Tag antibody
WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Host: Mouse Monoclonal 2C5 unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal FG4R unconjugated

anti-DYKDDDDK Tag antibody
D Host: Mouse Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB0493 unconjugated

anti-DYKDDDDK Tag antibody (HRP)
WB, ELISA, IP Host: Rabbit Polyclonal HRP

anti-DYKDDDDK Tag antibody
IP Host: Mouse Monoclonal 29E4-G7 unconjugated

anti-DYKDDDDK Tag antibody
WB, IP, IF Host: Mouse unconjugated

anti-DYKDDDDK Tag antibody
WB, ELISA, IP Host: Mouse Monoclonal 5A8E5 unconjugated

Application Notes

Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5 μL-2 μL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

DYKDDDDK Tag

Alternative Name

Flag Tag (DYKDDDDK Tag Products)

Target Type

Tag

Background

Background: Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Aliases: DDK tag, DYKDDDDK, Flag, Flag Tag

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