PPM1D antibody (AA 201-300) (HRP)

Catalog No. ABIN740424

The HRP-conjugated Rabbit Polyclonal anti-PPM1D antibody (ABIN740424) specifically detects PPM1D in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PPM1D antibody (AA 201-300) (HRP) (ABIN740424)

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1D antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPM1D Antibody (HRP)

Binding Specificity: AA 201-300

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PPM1D

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PPM1D

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Alternative Name: PPM1D

Background:

Synonyms: WIP1, PP2C-DELTA, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D

Background: Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.

Gene ID: 8493

UniProt: O15297

Pathways: p53 Signaling, Cell Division Cycle