MCL-1 antibody (pSer159, pThr163) (Cy3)

Catalog No. ABIN743741

The Rabbit Polyclonal anti-MCL-1 antibody is suitable to detect MCL-1 in samples from Human and Rat. It has been validated for WB, FACS, IF (cc) and IF (p).

Quick Overview for MCL-1 antibody (pSer159, pThr163) (Cy3) (ABIN743741)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MCL-1 Antibody (Cy3)

Binding Specificity: pSer159, pThr163

Specificity: These phosphorylation sites are homologous to Ser140 + Thr144 in Mouse and Ser139 + Thr143 in Rat.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Mcl 1 around the phosphorylation site of Ser159/Thr163

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: Mcl1 +

Background:

Synonyms: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, MCL1

Background: Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling