Human Adenovirus type 5 E1A (HAdV-5 E1A) (AA 181-280) antibody
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- Target
- Human Adenovirus type 5 E1A (HAdV-5 E1A)
- Binding Specificity
- AA 181-280
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Reactivity
- Adeno-Associated Virus 5 (AAV-5), Rat, Virus
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Rat, Virus
- Cross-Reactivity (Details)
- Adenovirus5
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Adenovirus 5 E1A
- Isotype
- IgG
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- Human Adenovirus type 5 E1A (HAdV-5 E1A)
- Target Type
- Viral Protein
- Background
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Synonyms: Adenovirus early E1A protein, Control protein E1A, E1A, Early E1A protein, Human adenovirus type 5, Human adenovirus C, pfam02703, E1A_ADE05.
Background: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
- UniProt
- P03255
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