Neurogranin antibody (AA 45-55)

Catalog No. ABIN769053

Product Details anti-Neurogranin Antibody

Target: Neurogranin (NRGN)

Binding Specificity: AA 45-55

Reactivity: Human, Mouse, Dog, Cow, Goat, Horse, Rabbit, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurogranin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NRGN / Neurogranin. Reported variants represent identical protein: NP_006167.1, NP_001119653.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Goat, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%) Marmoset, Mouse, Rat, Hamster, Elephant (91%) Arabidopsis (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KIKSGERGRKG from an internal region of human NRGN / Neurogranin (NP_006167.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Goat, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%), Marmoset, Mouse, Rat, Hamster, Elephant (91%), Arabidopsis (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 15  kDa band observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates (calculated MW of 7.6  kDa according to NP_006167.1). There is also an additional 16  kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15,274(3):1294-300, PMID: 9880498). Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Target Details for Neurogranin

Target: Neurogranin (NRGN)

Alternative Name: NRGN / Neurogranin (NRGN Products)

Synonyms: RC3 antibody, hng antibody, 0710001B06Rik antibody, AI838505 antibody, NG antibody, NG/RC3 antibody, Pss1 antibody, R75334 antibody, zgc:158686 antibody, neurogranin antibody, neurogranin (protein kinase C substrate, RC3) b antibody, NRGN antibody, Nrgn antibody, nrgnb antibody

Background: Name/Gene ID: NRGN

Synonyms: NRGN, Hng, Neurogranin, Ng, Protein kinase C substrate, RC3, Calmodulin-binding protein

Gene ID: 4900

NCBI Accession: NP_006167

UniProt: Q92686