CD33 antibody (CD33 Molecule) (APC)

Details for Product anti-CD33 Antibody No. ABIN775711, Supplier: Log in to see
Antigen
  • Siglec-3
  • gp67
  • SIGLEC-3
  • SIGLEC3
  • p67
  • CD33 molecule
  • CD33 antigen
  • CD33
  • Cd33
Reactivity
Human, Non-Human Primate
599
39
31
24
16
7
4
2
1
Host
Mouse
484
138
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD33 antibody is conjugated to APC
63
54
26
25
16
14
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14
12
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11
9
9
9
9
9
8
8
5
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4
4
4
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3
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2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
375
251
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116
105
84
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69
58
26
26
16
12
11
5
4
4
2
2
1
1
1
Options
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Immunogen Human AML cells
Clone WM53
Isotype IgG1
Specificity The mouse monoclonal antibody WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
HLDA IV, WS Code M-505
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD33 (CD33 Antibody Abstract)
Background CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Gene ID 945
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Garnache-Ottou, Chaperot, Biichle, Ferrand, Remy-Martin, Deconinck, de Tailly, Bulabois, Poulet, Kuhlein, Jacob, Salaun, Arock, Drenou, Schillinger, Seilles, Tiberghien, Bensa, Plumas, Saas: "Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." in: Blood, Vol. 105, Issue 3, pp. 1256-64, 2005 (PubMed).

Leone, Rutella, Bonanno, Abbate, Rebuzzi, Giovannini, Lombardi, Galiuto, Liuzzo, Andreotti, Lanza, Contemi, Leone, Crea: "Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function." in: European heart journal, Vol. 26, Issue 12, pp. 1196-204, 2005 (PubMed).

Schenk, Bouchon, Birrer, Colonna, Mueller: "Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 1, pp. 517-24, 2004 (PubMed).

Vitale, Romagnani, Puccetti, Olive, Costello, Chiossone, Pitto, Bacigalupo, Moretta, Mingari: "Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 10, pp. 5764-9, 2001 (PubMed).

Shin, Choi, Kim, Chung, Chung, Park, Jung, Kim, Park, Kim, Park, Min, Kim, Park: "Expression of leukemia-associated antigen, JL1, in bone marrow and thymus." in: The American journal of pathology, Vol. 158, Issue 4, pp. 1473-80, 2001 (PubMed).

Bradstock, Kirk, Grimsley, Kabral, Hughes: "Unusual immunophenotypes in acute leukaemias: incidence and clinical correlations." in: British journal of haematology, Vol. 72, Issue 4, pp. 512-8, 1989 (PubMed).

Favaloro, Bradstock, Kabral, Grimsley, Zowtyj, Zola et al.: "Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging ..." in: British journal of haematology, Vol. 69, Issue 2, pp. 163-71, 1988 (PubMed).

Favaloro, Bradstock, Kabral, Grimsley, Berndt: "Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33)." in: Disease markers, Vol. 5, Issue 4, pp. 215-25, 1988 (PubMed).