NMDAR2A antibody (pTyr1325)

Catalog No. ABIN784606

This Rabbit Polyclonal antibody specifically detects NMDAR2A in WB. It exhibits reactivity toward Rat and Mouse.

Quick Overview for NMDAR2A antibody (pTyr1325) (ABIN784606)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NMDAR2A Antibody

Binding Specificity: pTyr1325

Specificity: Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Cross-Reactivity (Details): Species reactivity (expected):Bovine and CanineSpecies reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDA NR2A.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Background: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A

Gene ID: 24409

NCBI Accession: NP_036705

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity